In addition to the RealAccurate® Quadruplex Corona-plus PCR Kit, PathoFinder launches the RealAccurate® Quadruplex SARS-CoV-2 PCR Kit (CE-IVD), which detects viral RNA from nasopharyngeal swabs and simultaneously targets the N gene and RdRp gene of the virus, allowing sensitive detection and confirmation of positive results at once.
The kit is validated on the five most prevalent real-time instruments, including LightCycler480 II (Roche), Rotor-Gene Q (Qiagen), MIC qPCR Cycler (BMS), CFX96 (Biorad) and Quantstudio 5 (Thermo Fisher Scientific).
PathoFinder has developed in combination with the RealAccuarate® Quadruplex SARS-CoV-2 PCR Kit, an unique extraction-free protocol. The robust composition of the RAQ SARS-CoV-2 PCR Kit allows replacement of a full nucleic acid extraction procedure by a simple heat-treatment. This decreases sample processing time and saves on nucleic acid extraction reagents and disposables.
Although, Ct/Cp values obtained with the heat-treated samples are slightly higher than those obtained with regular nucleic acid extraction, overall a nearly comparable sensitivity for SARS-CoV-2 detection is obtained using heat-treatment.
For more information go to the PathoFinder website.
Maastricht, June 2020