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Detection and identification of dermatophyte infections in nail, hair and skin samples

50/25 reactions

Products & Targets


PN-301  DermaGenius® 2.0 Nail Multiplex real-time PCR kit (50 rxn)  


  • Candida albicans
  • Trichophyton rubrum
  • Trichophyton interdigitale
  • Internal Control (IC)

PN-401/402 DermaGenius® 2.0/3.0 Complete Multiplex real-time PCR kit (50 rxn)


  • Scopulariopsis brevicaulis*
  • Candida paraosilosis*
  • Nannizia gypsea*
  • Candida albicans
  • Trichophyton mentagrophytes
  • Trichophyton interdigitale
  • Trichophyton rubrum / soudanense
  • Trichophyton tonsurans
  • Trichophyton violaceum
  • Microsporum canis
  • Microsporum audouinii
  • Epidermophyton floccosum
  • Trichophyton benhamiae
  • Trichophyton verrucosum
  • Pan-dermatophyte*
  • Internal Control (IC)

*Addtional targets offered in DermaGenius® 3.0 Complete Multiplex real-time PCR kit

PN-303  DermaGenius® Resistance Multiplex real-time PCR kit (25 rxn)  


  • Trichophyton rubrum/soudanense
  • Trichophyton interdigitale/mentagrophytes
  • Trichophyton mentagrophytes (ITS type IV)
  • Trichophyton tonsurans
  • Trichophyton violaceum
  • Trichophyton quinckeanum/schoenleinii
  • SQLE: Leu393Phe, Phe397Leu, Leu393Ser, Phe397Ile, Phe397Val
  • Internal Control (IC)

Features and benefits

  • Aids in the diagnosis of dermatophytosis (superficial fungal infections)
  • Detects > 95% of the clinically most relevant dermatophytes
  • Dermatophyte species identification, enabling: Targeted Treatment
  • Detection of mutations in the squalene epoxidase (SQLE) gene (D. Resistance)
  • Differentiation between wildtype and resistant SQLE strains (D. Resistance)
  • Detection in nail, skin and hair samples
  • Sample-to-result in less than 3 hours
  • Higher sensitivity: up to 20% more positive results compared to culture
  • Identification of source of infection
  • Assistance in Outbreak management
  • Including Pan-dermatophyte mix

Real-time PCR instruments

  • LightCycler 480 II (Roche)
  • Rotor-Gene Q (Qiagen)
  • CFX96 (Biorad)
  • QuantStudio 5 (Thermo Fisher Scientific)
  • Mic qPCR (BMS)

Diagnostic specimens

  • Nail, hair and skin samples
  • Paraffin embedded samples (FFPE)


  • Validated on clinical samples
  • Internal Control (IC) included
  • Positive Controls (PCs) included
  • Validated on nail, hair and skin samples
  • Validated on EQA programmes of QCMD
  • CE-IVD marked


Dermatophytes represent the most numerous and widespread group of all mycoses and have the capacity to invade keratinized tissue of humans and animals to facilitate infections that are generally restricted to the corneocytes of the skin, hair and nails.
Dermatophytes are classified in three genera: Epidermophyton, Microsporum and Trichophyton. Diagnosis of these infections is currently based on microscopy or histology associated with culture on specific agar media. However, direct microscopy lacks specificity and culturing has a long turn-around-time of 2-4 weeks and is prone to contamination. These limitations can be prevented by the use of molecular diagnostics.


Hayette et al., Medical Mycology, 2018; 277–283

Trovato et al., Med Mycol Case Rep., 2019; 24: 72–74

Singh et al., Fungal Genetics and Biology, Vol 133, 2019

Singh et al, Mycoses 2021 Jul;64(7) 721-726

PathoNostics Extraction kit

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PN-502 PathoNostics Extraction kit (50 rxn)
  • DNA extraction from nail, hair, skin samples in 20 min


  • Solution A (lysis buffer)
  • Solution B (neutralization buffer)


  • Validated on fungal cultures
  • Validated on nail, hair, skin samples
  • CE-IVD marked

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